Lipid oxidation was expressed as thiobarbituric acid reactive substances (TBARS) (mg malonaldehyde/kg sample), as determined by Botsoglou et al. (1994), using a UV2 spectrophotometer
The pH was measured using a micropH 2001 pHmeter , and a combined electrode Cat. No. 52-22, to remove the possible contribution to microbiological or colour changes caused by to variations in the pH of the patties.