Briefly, a loopful of the
culture to be evaluated was inoculated on agar plates with the
detection medium (2% (w/v) sucrose (Merck), 0.5% (w/v) yeast
extract (Britania), 2% (w/v) peptone (Britania), 1.5% (w/v) agar (Britania)
autoclaved and supplemented with 1% (w/v) gelatin) and
incubated at 37 C for 48 h.