From two fractions of 50 ll protein samples, one aliquot was treated with 2 ml of 2.0 N HCl (control) and the other was treated with 2.0 ml of 10 mM 2,4-dinitrophenylhydrazine (DNPH) in 2.0 N HCl for 1 h at room temperature.
From two fractions of 50 ll protein samples,one aliquot was treated with 2 ml of 2.0 N HCl (control) and theother was treated with 2.0 ml of 10 mM 2,4-dinitrophenylhydrazine (DNPH) in 2.0 N HCl for 1 h at room temperature.