2.2.1. Extraction of bromelain from pineapple core
To obtain enzyme extract pineapple core was crushed with
buffer containing 1% PVP in blender.The core to buffer ratio was
maintained as 1:1 w/v.After filtrationwith cheese cloth extract
was centrifuged at 10,000 g for 20 min incentrifuge (REMI compu-
fuge PR24, REMI Instruments , Vasai,India)to get clear extract.The
extract was stored at 5–8 C until use.To check the effect of buffer
on bromelain extraction,fivedifferent buffers were used.The com-
binations of salts used in the preparation of buffers were (a)potas-
sium phosphate buffer (P.P: potassium phosphate monobasic and
dibasic), (b)sodium–potassium phosphate buffer (S.P.P:sodium
phosphate dibasic and potassium phosphate monobasic),(c)
sodium phosphate buffer (S.P: sodium phosphate monobasic and
dibasic), (d)potassium-hydroxide buffer (P.H:potassium phos-
phate dibasic and sodium hydroxide),(e)citrate–phosphate buffer
(C.P: citric acid monohydrate and sodium phosphate monobasi c).
To check the effect of maturity offruit 0.01 M Sodium phosphate
buffer (pH 6.5)with 1%PVP was used.
4. Conclusion
For both Nang Lae and Phu Lae pineapple, the peel is the
largest waste portion followed by the core, stem, and crown.
The crown extract of both cultivars contained bromelain as
a major enzyme with the MW ~28kDa. Most of the extracts
exhibited the highest caseinolytic activity at a pH of around
7.0, whereas those of stem extracts were 8.0. All extracts of
Nang hae pineapple produced the highest activity at 50 °C. For
Phu Lae extracts, the highest activity occurred at 60 =C. The
peel seems to have the most promise for bromelain extraction
because it accounts for the largest waste proportion. The
pineapple waste residues after bromelain extraction contain
high amount of fiber. It would be useful to suggest further
application of these residues for paper and paper board productions.
2.2.1. Extraction of bromelain from pineapple core
To obtain enzyme extract pineapple core was crushed with
buffer containing 1% PVP in blender.The core to buffer ratio was
maintained as 1:1 w/v.After filtrationwith cheese cloth extract
was centrifuged at 10,000 g for 20 min incentrifuge (REMI compu-
fuge PR24, REMI Instruments , Vasai,India)to get clear extract.The
extract was stored at 5–8 C until use.To check the effect of buffer
on bromelain extraction,fivedifferent buffers were used.The com-
binations of salts used in the preparation of buffers were (a)potas-
sium phosphate buffer (P.P: potassium phosphate monobasic and
dibasic), (b)sodium–potassium phosphate buffer (S.P.P:sodium
phosphate dibasic and potassium phosphate monobasic),(c)
sodium phosphate buffer (S.P: sodium phosphate monobasic and
dibasic), (d)potassium-hydroxide buffer (P.H:potassium phos-
phate dibasic and sodium hydroxide),(e)citrate–phosphate buffer
(C.P: citric acid monohydrate and sodium phosphate monobasi c).
To check the effect of maturity offruit 0.01 M Sodium phosphate
buffer (pH 6.5)with 1%PVP was used.
4. Conclusion
For both Nang Lae and Phu Lae pineapple, the peel is the
largest waste portion followed by the core, stem, and crown.
The crown extract of both cultivars contained bromelain as
a major enzyme with the MW ~28kDa. Most of the extracts
exhibited the highest caseinolytic activity at a pH of around
7.0, whereas those of stem extracts were 8.0. All extracts of
Nang hae pineapple produced the highest activity at 50 °C. For
Phu Lae extracts, the highest activity occurred at 60 =C. The
peel seems to have the most promise for bromelain extraction
because it accounts for the largest waste proportion. The
pineapple waste residues after bromelain extraction contain
high amount of fiber. It would be useful to suggest further
application of these residues for paper and paper board productions.
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