3. Results and discussion
In a first step, experiments were designed to evaluate the effects of sugarcane bagasse pretreatment, substrate particle size, and cultivation method on endoglucanase production in Erlenmeyer flask cultivations. In a second step, the effects of agitation speed and cultivation method on (hemi) cellulolytic enzymes production were evaluated in stirred tank bioreactor cultivations. The effects of biomass pretreatment and pH control were then evaluated in STB cultivations. Once the most suitable bioprocess parameters had been selected, the influence of biomass particle size on (hemi) cellulolytic enzymes production was also evaluated in STB cultivations. All experiments were replicated, and the results are presented as means ± standard deviations.