3.2. Detection of CBV proteins in the
hemolymph, gill, and in primary cell cultures
Western blot analysis of CBV-containinghemolymph and gill samples obtained from timecourse
experiments using experimentally infected
shrimps showed the appearance of the three
prominent CBV nucleocapsid bands at 43 and 41
h p.i., respectively (Fig. 3). The shrimp did not
show any overt symptoms of disease until 72 h
p.i.
Western blot analysis of CBV-infected primary
lymphoid cell cultures showed similar results. The
three prominent bands of the virus were easily
visible as early as 4 days