Developed bioformulations were evaluated for their effectiveness
in controlling sugar beet seedling damping-off disease in a
greenhouse experiment conducted in a completely randomized
(CR) design with ten treatments (nine bioformulations and an untreated
control) each with four replications (Table 2). Each replicate
consisted of a plastic pot containing 3 kg of pasteurized field soil
pre-inoculated with R. solani inoculum at the rate of 1% (w/w) and
20 sugar beet seeds (Shirin variety) coated with each powdery
bioformulation. For preparation of R. solani inoculum, it was grown
on autoclaved wheat grains for 3 weeks until the fungal mycelia
covered the grain surface. It was then dried out, blended, and used
as inoculum in the greenhouse experiment as described above.
Pots were placed in a greenhouse with 12 h light photo period
and were watered as needed. The number of survived sugar beet
seedlings (stand)was counted in each pot 60 days after sowing. The
efficacy of bioformulations in controlling sugar beet seedling
damping-off disease was then determined by comparing the