For coating eggshells with chitosan, solutions containing 0.25%, 1%
and 2% (w/v) of the selected chitosan were prepared by dissolving
chitosan in 1% (v/v) acetic acid and 2% (v/v) glycerol (plasticizer) and
then bringing this solution to pH 5.0 by adding sodium hydroxide. To
promote dissolution of the 2% chitosan solution, this was performed in
a water bath at 50 °C