To test the experimental reproducibility, six runs were carried out with 0.5–1 mm dried carrot particles at 55 °C, 55.1 MPa, 2.5% canola oil concentration and 1 L/min CO2 flow rate. The coefficient of variation for the total amount of oil collected was 5.6%. Compared with the amount of canola oil introduced into the system as a co-solvent (at CO2 flow rate of 1 L/min, ca. 12 g canola oil was pumped into the system at 2.5% oil addition and ca. 24 g at 5% oil addition), the amount of crude oil in the 2 g dry carrot sample is extremely small (approximately 0.04 g). Therefore, the total amount of oil collected after extraction with SC-CO2 + canola oil was mainly the canola oil added to the system as a co-solvent, plus a very small amount of crude carrot oil extracted from the feed material. However, it was not possible to separate the crude carrot oil from the canola oil added as co-solvent. The total amount of oil collected after the 4 h SC-CO2 extraction with canola oil addition at 2.5% level was 2.39–7.51 g, while that at 5% level was 1.46–9.69 g. The total amount of oil collected in 4 h increased with pressure at both levels of canola oil addition and the increase became more substantial at higher temperatures reflecting the changes in canola oil solubility in SC-CO2 and higher level of canola oil addition (Fig. 2).