Resistant starch (Hi maize) and chitosan at concentrations of 1% and 0.4% were added to the microencapsulationof Lactobacillus acidophilus in alginate beads by extrusion technique. Moist and freeze-driedmicroparticles were analyzed. The addition of prebiotics and chitosan increased the size of the moistparticles, whose diameter was 70.37 mm, while the diameter of the microparticles containing alginatealone was 55.13 mm. In contrast, the freeze-dried microparticles of alginate and alginate þ HieMaize þ chitosan had diameters of 114.51 mm and 112.50 mm, respectively. Both Hiemaize and chitosanprovided better protection of probiotics after exposure of the moist microparticles to simulated gastricand intestinal juice, with counts of 6.35 log CFU g1, while lower counts were observed for the freezedriedmicrocapsules. Regarding the viability of the probiotic culture during the storage periods andtemperatures, all treatments were viable, with suitable values to confer the probiotic effects (<6 log CFUg1), with counts up to 6 logs for at least 30 days for the microparticles stored in the freeze-dried form,and 135 days in the moist form, both under storage at room temperature (25 C).
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