A number of factors can affect the reaction process
leading to the ultimate formation of methane and
carbon dioxide. The particulate nature, lignin, cellulose
and hemicelluloses content of biomass may affect the
overall reaction kinetics leading to biogas formation.
Other factors that may affect the biogas yield include,
low pH due to accumulation of by-products formed
during biodegradation, C/N ratio (carbon to nitrogen
ratio), temperature, hydraulic retention time, and
loading rate [4]. AD can be completed at psychrophilic