Confirmation experiments
The confirmatory experiments were conducted with the parameters
as suggested by the numerical modeling (suggested solutions)
and keeping the temperature and pH at 60 ◦C and 4.8 to support the
optimized data as given under optimized condition. The effect of
temperature, pH, enzyme and substrate concentration were studied
to support the results, and data in accordance with the results
obtained from optimized conditions and found that the results are
in accordance of the RSM study. There were very small differences
in the predicted response (11.26 U/ml) and the activity observed
(13.05) during the experiments when carried out at temperature
60 ◦C, pH 4.8, enzyme concentration 840l/ml and substrate concentration
149.0 mg/ml.