For the extraction of β-glucans from the yeast cell wall, various methologies have been proposed. All the published methods and patents for β-glucan production are based on the same idea: at first, yeast cell walls have to be obtained and then the cell walls must be gradually peeled for the removal of the other cell wall components. The differences are found in the applied technique for the yeast cell disruption and the extraction process for β-glucan production. The choice of the suitable method depends on a combination of parameters such as the further use of produced soluble or insoluble β-glucan (incorporation in medicines and functional foods), the purity, the yield, the cost, the time, the equipment needed, etc. Wine and brewing industrial wastes can comprise a new source for yeast biomass material as the wine lees and the spent brewer's yeast remaining after the completion of the alcoholic fermentation can be used for yeast β-glucan production.