solubilization by SDS overnight at
65 C [22] or for 5 h at 45 C [1] with dithiothreitol at pH 9.0–9.5
and urea or 2-mercaptoethanol for 16–72 h [18,23].
Since our purpose was to detect UVR-induced changes in
extracted and residual soluble peptides and proteins upon UV irradiation
of hair we preferred mild experimental conditions in order
to get the material unaffected by reducing agents and long incubation
times. We used carbonate buffer solution (pH 10.5), and it was
shown earlier that visible and UV light increase hair alkaline solubility
[24].
Our data have shown growth in proteins elution by 142%