Compared with mesophilic pullulanases, thermophilic pullulanases
possess higher optimal temperatures (Fig. 1). In this study,
the optimal temperature of Bapul-TSwashigher than that of the WT
enzyme. Seemingly, protein thermostability was positively related to the optimal temperature of protein. The enhanced optimum
temperature and improved thermostability of Bapul-TS made this
enzyme more useful than the WT enzyme. In addition, excellent
pH stability made Bapul-TS more suitable than most thermophilic
pullulanases for saccharification.