Data on mobility, viability and osmotic resistance of avian sperm cells in control and irradiated samples are showed in Fig. 1, Fig. 2 and Fig. 3, respectively. The irradiation was significantly effective in increasing sperm motility in the turkey semen (0.228 ± 0.01 compared with 0.294 ± 0.02). In the other species was: 0.170 ± 0.05 compared with 0.277 ± 0.02; 0.154 ± 0.02 compared with 0.155 ± 0.05; 0.228 ± 0.01 compared with 0.294 ± 0.02 for chicken and pheasant spermatozoa, respectively. This bio-stimulation also resulted in a significant increase in the COX activity (Fig. 4) in pheasant and turkey sperm (+135 ± 4.3 and +116 ± 4.3%, respectively). In contrast, viability and osmotic-resistance of avian spermatozoa were not significantly affected by laser irradiation.