The extraction of HESCs from inner cell mass for research purpose leads to the destruction of the embryo. The major source of human embryonic stem cell tissues are the spare or supernumerary embryos created during in vitro fertilization as a part of infertility treatment. The other source is creating embryos with somatic cell nuclear transfer techniques (SCNT). The legislation of most countries including India allows use of spare or supernumerary embryos either fresh or frozen created during in-vitro fertilization.