Blue containing 0.1 M aluminum nitrate, and then destained
in a 10% acetic acid solution. The stained gel was scanned
using a GS-710 (Bio-Rad Laboratories Inc, Hercules, CA,
USA) densitometer at an optical resolution of 63.5 m/pixel
and then analyzed by Image Master 2D Platinum v5.0 (GE
Healthcare, formerly Amersham Biosciences). The relative
phosvitin composition in the protein solution was estimated
from band intensity (pixel intensityband area).