One ml of the rinse water from the sample was placed onto the center of the bottom film and covered carefully avoiding entrapment of air bubbles and the plates from all samples incubated for 24 ± 2 hrs at 35°C. Colony counts for each petrifilm were done the following day and the microbial count results converted to the base 10 logarithm of the number of colony forming units per ml (cfu/ml) rinse water obtained from the samples.