A dried tomato by-product sample including tomato seed and a
mixture of tomato peel and seed sample (37:63 w/w) was obtained
from Kagome Co. Ltd. Japan. Initially, the sample was ground using
a coffee grinder and sieved to 1.05 ± 0.10 mm. Standard lycopene
and b-carotene, HPLC grade of acetonitrile, methanol, and 2-propanol
were obtained from Wako Pure Chemical Industries Ltd., Japan,
and CO2 from Uchimura Co., Japan.