For thermophilic aerobic bacteria growth, such as Geobacillus stearothermophilus, 10 briks of each treatment at day 1 and at day 130 of storage were placed at 55 °C for 10 days. Total mesophilic aerobic bacteria were determined on PCA medium (Oxoid Ltd., Basingstoke, Hampshire, UK) and total thermophilic aerobic bacteria on TSAmedium (Oxoid).