the aim of study was to establish a reproducible protocol using the methodology of hyaline zones around the colony on specific agar plates for phospholipase and proteinase production
this was an in vitro double-blind experiment in which the dependent variables were the enzymatic activity measurement for the production of phospholipase and the production of secreted aspartyl proteinases
three independent variables give rise to different measurement protocols
all measurements were carried out at two different moments by four examiners e1 e2 e3 e4
he minimum sample size was 30 candida albicans clinical isolatese. specific agar plates for phospholipase and asps production were prepared according the literature the intra-and inter-examiner reproducibililty for each protocol was estimated using the intraclass correlation coefficient and is confidence interval based on the results obtained for both phospholipase and asps there appear to be no consensus on the protocol chosen for each particular examiner