White bread contained the lowest amounts of freeumami amino acids and umami 50-nucleotides and showed the lowest EUC value. Incorporating 5% mushroommycelia into the bread formula did not adversely affect the texture profile of the bread. However,incorporating 5% mushroom mycelia into the bread formula did lower bread’s acceptability. After baking,mycelium-supplemented bread still contained substantial amounts of c-aminobutyric acid and ergothioneine(0.23–0.86 and 0.79–2.10 mg/g dry matter, respectively). Overall, mushroom mycelium could beincorporated into bread to provide its beneficial health effects.