The IgG fraction of the MoAb was precipitated by 50% saturated ammonium sulfate, dialyzed against an excess of acetate buffer and purified by SP-Sepharose ion-exchange chromatography (Amersham Pharmacia Biotech AB, Uppsala, Sweden).
The IgG fraction of the MoAb was precipitated by 50% saturated ammonium sulfate, dialyzed against an excess of acetatebuffer and purified by SP-Sepharose ion-exchange chromatography (Amersham Pharmacia Biotech AB, Uppsala, Sweden).