Pectinase
The extraction yield of soluble solids significantly depended (P > 0.05) on treatment time, as well as the pectinase concentration. Pectinase addition at level 50 U/100 g increased the extraction yield of soluble solids by 8.48% after 40 min. This was related to degradation of the pectin in the plant cell wall matrix and in the middle lamella into galacturonic acid units and sugars
The yield of soluble solids increased with increase in enzyme concentration (Table 4). However, the addition of the highest concentration of pectinase (100 U of pectinase/100 g) presented no significant change (P > 0.05) on the extraction yield of soluble solids compared with 50 U of pectinase/100 g. The turbidity was significantly affected (P < 0.05) by time and enzyme concentration. The turbidity decreased with increase in enzyme concentration. In fact, the turbidity falls of 76.03%, 80.14%, 83.73% and of 84.66% for 5 U, 10 U, 50 U and 100 U of pectinase after 100 min, respectively (data not shown). In the non-enzyme-treated control, at least 180 min were necessary to decrease the turbidity to a comparable level to that obtained with enzyme treatment.
The reduction of the turbidity is probably due to pectin hydrolysis in the plant cell wall matrix and in the middle lamella allowing liberation of galacturonic acid
PectinaseThe extraction yield of soluble solids significantly depended (P > 0.05) on treatment time, as well as the pectinase concentration. Pectinase addition at level 50 U/100 g increased the extraction yield of soluble solids by 8.48% after 40 min. This was related to degradation of the pectin in the plant cell wall matrix and in the middle lamella into galacturonic acid units and sugars The yield of soluble solids increased with increase in enzyme concentration (Table 4). However, the addition of the highest concentration of pectinase (100 U of pectinase/100 g) presented no significant change (P > 0.05) on the extraction yield of soluble solids compared with 50 U of pectinase/100 g. The turbidity was significantly affected (P < 0.05) by time and enzyme concentration. The turbidity decreased with increase in enzyme concentration. In fact, the turbidity falls of 76.03%, 80.14%, 83.73% and of 84.66% for 5 U, 10 U, 50 U and 100 U of pectinase after 100 min, respectively (data not shown). In the non-enzyme-treated control, at least 180 min were necessary to decrease the turbidity to a comparable level to that obtained with enzyme treatment.The reduction of the turbidity is probably due to pectin hydrolysis in the plant cell wall matrix and in the middle lamella allowing liberation of galacturonic acid
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