Based on a comparison of partial 16S rRNA gene sequences
(Goto et al. 2000, 2002), strain Shu-P-Ggiii25-2T was
placed in the Amphibacillus–Halolactobacillus cluster.
However, the sequences of the novel strain were found to
be distinct from previously described species of both of
these genera. The 16S rRNA gene sequence was determined
using a 16S rRNA gene kit following the manufacturer’s
protocols (Applied Biosystems). The 16S rRNA gene
sequence of strain Shu-P-Ggiii25-2T was used for BLAST
searches via the NCBI (National Center for Biotechnology
Information). The sequences obtained were aligned by
using the CLUSTAL_X software package (Thompson et al.,
1997) and evolutionary distances and Knuc values (Kimura,
1980) were generated. Alignment gaps and ambiguous
bases were not considered when the 1350 bases of the 16S