Although using chitosan and oregano showed substantial effect on the growth of decomposer microorganisms, chitosan individually as a single dose was not effective. Additionally, chitosan was not effective against enterobacteriaceae count and E. coli O157: H7 for all storage temperatures and in the sample inoculated with L. monocytogenes; chitosan was only effective at 3 and 8◦C, in the absence of oregano EO. Tsai et al (2006) demonstrated that chitosan is more effective at lower temperatures