To determine the specificity of the primers and probes, multiplex real-time PCR as described below was done with the 28 strains in Table 1, including 13 target and 15 non-target strains. These experiments were done twice.
To determine the specificity of the primers and probes, multiplexreal-time PCR as described below was done with the 28 strainsin Table 1, including 13 target and 15 non-target strains. Theseexperiments were done twice.