Total soluble proteins (TSP) were extracted from the mature
leaves of eight-week-old homoplasmic and non-transformed
plants. Ten regenerated transplatomic plants and three leaves of
every plant were collected for analysis. 200 mg of leaves were
ground in liquid nitrogen and resuspended in 1 ml of protein
extraction buffer [0.2 M Tris-HCI (pH 8.0), 5 mM ethylene diamine
tetra acetic acid (EDTA), 100 mg Sucrose, 0.1 mM 2-
Mercapthoethanole].