As shown in Table 2, the difference
of ethanol concentration in R1 was not significant when the
temperature increased from 30 C to 35 C (P ¼ 0.057). The ethanol
concentration in R2 at 33 C was not significantly different from
that at 30 C (P ¼ 0.414), while it significantly decreased to
75.2 g L1 at 35 C (P ¼ 0.016). Thus, the temperature in R2 should
be controlled at 33 C