The evaluation was conducted over two well-defined periods. During the first period, from 01 January 2010 to 31 December 2012, only calcium carbonate (600 mg/day/elemental calcium) was used. During the second period from 01 January 2013 to 31 January 2014, the pregnant women began to receive CC+CLA (600 mg/day/elemental calcium and 450 mg/day of conjugated linoleic acid).