Figure 3 A detailed analysis of lens regeneration, showing lens proliferation and crystalline synthesis. All lentectomies were performed
at stage 44 + 8 days. The first column shows bright field sections through the heads and the eyes. The second column shows bright field
sections with BrdU staining (green) and g-crystallin staining (red) X10. The third column shows higher magnification images to depict better the
regenerating lenses. The different rows represent samples at different time points after lentectomy. Note that the regenerating lens comes from
the dorsal iris (the case at 6 hours) or the ventral iris (case at 48 hours). The regenerating lens is obvious at 6 hours post-lentectomy and starts
organizing well with proliferating lens epithelium at the anterior (arrows) and differentiating lens fibers at the posterior (arrowheads) as soon as
12 hours post-lentectomy. The proliferating cells in the ciliary margin (open arrows) are retina stem/progenitor cells that contribute to the
growing retina.