Chemical analysis
Dry matter content of the PV and CS were determined by
drying samples in an oven at 65°C for 48 h. The dried
samples were ground for chemical composition analysis.
NDF and ADF were determined in the method described
by van Soest and Wine (1991). Water soluble carbohydrate
(WSC) were quantified in the enthrone method reported
by Yemm and Willis (1954). CP was analyzed using
the Kjeldahl method (AOAC 1990). Crude ash (CA) was
obtained after 5 h in a muffle furnace at 550°C (Filya 2003).