The amount of fluorescent pseudomonads in the plant cultivation
system is shown in Fig. 1 and after six days there was significant
difference in the amount of fluorescent pseudomonads
compared with the control treatment (F ¼ 10.62; df1 ¼4, df2 ¼ 10;
p ¼ 0.001). In the control treatment the average amount of fluorescent
pseudomonads over time, expressed as mean SD, was log
2.8 0.2 cfu mL1 of nutrient solution. When a high concentration
of washed cells of P. koreensis 2.74 was added to the system a rapid
decrease in fluorescent pseudomonads was observed, from log
7.5 0.1 cfu mL1 1 h after addition of the cells to log
5.1 0.1 cfu mL1 after 24 h. After six days there was no significant
difference in the amount of fluorescent pseudomonads compared
with the control treatment. A very similar patternwas observed for
the treatment receiving a high amount of cells directly in KB broth.
A comparable trend was found when a lower concentration of
washed cells was added to the system, namely a rapid decrease
from log 5.2 0.2 cfu mL1 to log 3.7 0.1 cfu mL1 during the first
24 h, while after six days no significant difference could be found
compared with the control. Applying the cells and crude broth,
when a minimal medium with rapeseed oil as a carbon source was
used for cultivation, resulted in a rapid decrease in the amount of
fluorescent pseudomonads during the first 24 h, from log
6.3 0.2 cfu mL1 to log 4.4 0.1 cfu mL1. However, after six days
the amount of fluorescent pseudomonads in this treatment (log
3.7 0.1 cfu mL1) was significantly higher than in the control
treatment (2.7 0.1 cfu mL1).
The amount of fluorescent pseudomonads in the plant cultivation
system is shown in Fig. 1 and after six days there was significant
difference in the amount of fluorescent pseudomonads
compared with the control treatment (F ¼ 10.62; df1 ¼4, df2 ¼ 10;
p ¼ 0.001). In the control treatment the average amount of fluorescent
pseudomonads over time, expressed as mean SD, was log
2.8 0.2 cfu mL1 of nutrient solution. When a high concentration
of washed cells of P. koreensis 2.74 was added to the system a rapid
decrease in fluorescent pseudomonads was observed, from log
7.5 0.1 cfu mL1 1 h after addition of the cells to log
5.1 0.1 cfu mL1 after 24 h. After six days there was no significant
difference in the amount of fluorescent pseudomonads compared
with the control treatment. A very similar patternwas observed for
the treatment receiving a high amount of cells directly in KB broth.
A comparable trend was found when a lower concentration of
washed cells was added to the system, namely a rapid decrease
from log 5.2 0.2 cfu mL1 to log 3.7 0.1 cfu mL1 during the first
24 h, while after six days no significant difference could be found
compared with the control. Applying the cells and crude broth,
when a minimal medium with rapeseed oil as a carbon source was
used for cultivation, resulted in a rapid decrease in the amount of
fluorescent pseudomonads during the first 24 h, from log
6.3 0.2 cfu mL1 to log 4.4 0.1 cfu mL1. However, after six days
the amount of fluorescent pseudomonads in this treatment (log
3.7 0.1 cfu mL1) was significantly higher than in the control
treatment (2.7 0.1 cfu mL1).
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