Based on the literature on the success of rooting, there
are two suitable methods for in vitro rooting of microshoots.
They can be performed either by culturing microshoots
on media with low concentrations of auxin or treating
the microshoots with high concentration of auxin solution
followed by transfer to an auxin-free solid medium. Usually,
1/2 strength MS medium supplemented with IBA (0.5–
8 mg/ L) is used on in vitro rooting of tea [31, 32]. In order to
improve in vitro rooting of tea, we treated the healthy shoots
(2-3 cm height) with different high concentrations of IBA
for 30 minutes before transferring to half strength MS basal
medium without growth regulators. There was a significant
difference between the various concentrations of IBA with
300 mg/L being the most suitable concentration resulting in
72.3% of rooted microshoots. This finding corroborates the
ideas of Das et al. [33] and Jain et al. [34], who suggested
treating microshoots at the cut end with high concentrations
of IBA and transferring directly in the soil mix