Dry matter intake did not differ (P = 0.19) among treatments by betaine supplementation. In agreement, previous studies reported that DMI was unaffected by rumen-protected betaine (Davidson et al., 2008) and rumen-unprotected betaine (Wang et al., 2010) supplementations in lactating dairy cows. However, Löest et al. (2002) observed an increase in DMI in steers when rumen-unprotected betaine was top-dressed on the upper one-fourth of the ration, which might have increased palatability of the ration and stimulated intake