The antibacterial activity of silver nanoparticles was investigated against various human pathogenic organisms using well diffusion method. The synthesized silver nanoparticles were more effective against gram positive bacterial strain than the gram negative bacteria. Silver nanoparticles have antimicrobial effect on Staphylococcus aureus. The reason for different sensitivity between Gram-positive and Gram-negative bacteria could be explain to the morphological differences between these microorganisms [29]. Gram-negative bacteria, having an outer polysaccharide membrane and them carrying the structural lipopolysaccharide components. This makes the cell wall impermeable to lipophilic solutes, The Gram-positive strains should more susceptible having only an outer peptid oglycan layer which is not an effective permeability barrier. These results suggest the Biosynthesized silver nanoparticles are highly antagonistic in nature and they showed high antibacterial activity because silver nanoparticles allowed them to easily interact with other particles and increased their antibacterial activity due to its small size compared to the chemical synthesized components.