n contrast, Fig. 5 shows that the Chlorophyll content was easily removed when the transesterification reaction of the microalgal oil in hexane was conducted at 45 C for 15 min with a hexane-to-methanol ratio of 6:1. This condition is identical to a methanol to microalgae oil weight ratio of 5.22:1 and a catalyst loading of 0.033 g-NaOH/g-microalgae oil (see the TSBP set in Fig. 5)