Carotenoprotein(50g)wasextractedbyhomogenisingthe
sample with50mlofacetone.Theextractwas filtered usinga
filter paperNo.1.Theresiduewasrepeatedlyextractedwith
fresh solventuntilitwascolourless.Theextractswerepooled
together andwerephase-separatedwithanequalquantityof
hexane. Thehexaneextractwasrepeatedlywashedwithan
equal quantityof0.1%salinetoremovetracesofacetoneif
any. Theextractwasdriedwith12.5gofsodiumsulphate,
filtered andthenevaporatedundervacuumat40 1C usinga
rotary evaporator.Theresidualsolventwasremovedby
flushing withnitrogengas.Theextractedcarotenoidswere
subjected todeterminations.