nanofiltration, respectively (Fig. 3 first group of bars). All free
amino acids were bound to the resin after incubation for 30 min,
while 58% of total mass and 80% of soluble sugars remained in
the soluble phase (Fig. 3, second group of bars). Washes, using
pH 2 water, allowed for removal of the remaining sugars and
unidentified components bound to the resin (Fig. 3, third group
of bars) without any losses of bound amino acids (Table 3). The
resin was also washed using 0.5 N NH4OH in order to remove
poorly bound acidic amino acids (Fig. 3, fourth group of bars).
Aspartic and glutamic acid accounted for 85% of the eluted amino
acids (Table 2). Finally, arginine was recovered from the resin by
washing three times with 7.5 N NH4OH (Fig. 3, fifth group of bars,
Tables 2 and 3).