Briefly, 120 L of diluted sample were added to 2.4 mL of phosphate buffer (pH 7.4)
375 L of bidistilled water, 30 L of diluted R-PE and 75 L of ABAP
the reaction kinetics at 38°C were recorded for 45 min (or more, if necessary) by a LS-55 luminescence spectrometer (Perkin Elmer, Wellesley, MA).