The compounds were separated on a Waters Acquity BEH C18 column (50 2.1 mm, particle size 1.7 lm) using 0.5% formic acid in water (Solvent A) and a mixture of acetonitrile, 2-propanol and formic acid in the ratio of 94.5:5:0.5 (solvent B)
The compoundswere separated on a Waters Acquity BEH C18 column(50 2.1 mm, particle size 1.7 lm) using 0.5% formic acid in water(Solvent A) and a mixture of acetonitrile, 2-propanol and formicacid in the ratio of 94.5:5:0.5 (solvent B)