Image analysis
Image acquisition was performed with an ImageScanner II
device (GE Healthcare, Uppsala, Sweden). Intensity calibration was
carried out using an intensity step wedge prior to the image capture.
The TotalLabTM v2.01 software (Nonlinear Dynamics Ltd., NC, USA)
was used for image analysis. Spots were automatically detected
and matched. Each spot volume was determined densitometrically
and processed by background subtraction. Spot volumes of all gels
were normalized by dividing the raw quantity of each spot by the
Fig. 1. SDS-PAGE images of intact blood plasma (A) and protein fractions that precipitate at 0–40% and 40–50% saturation (B). Albumin band is highlighted with an
arrow.
total quantity of all valid spots in that gel. Finally, gel images were
manually annotated.