An aliquot (filtrate) of 50 ml of each sample was mixed with 100ml of 0.5 N perchloric acid in a 200 ml flask and allowed to stand for 5 min at room temperature to precipitate protein.
An aliquot (filtrate) of 50 ml of each sample was mixedwith 100ml of 0.5 N perchloric acid in a 200 ml flask and allowed tostand for 5 min at room temperature to precipitate protein.