3.2. Evaluation of growth medium
To obtain a simple and inexpensive growth medium as well as
high biomass production, plain seawater and enriched seawater
media were evaluated for algal culture. A significant growth of
Thalassiosira sp. cells in plain seawater medium until 9th day
was not observed under the conditions of the experiment
(Fig. 1A). This suggested that the plain seawater medium was
insufficient to grow Thalassiosira sp. However, in Walne and enriched
seawater media, the Thalassiosira sp. cells needed only a
day to adapt in the medium, then grew well until reaching the late
logarithmic phase on the 6th day, and afterwards fell (Fig. 1A).