The primer PBZAC7NcoI (50-TACAAGCCATGGCACCACCAAAGCG-30) was
used to include a NcoI restriction site (underlined) into the
start codon of the rep gene and the primer PBZAR1076XhoI
(50-TGCTACTCGAGAAATTCGGATCT-30) was used to
include a XhoI restriction site (underlined) downstream the
stop codon of the ren gene.