Hardening and transplantation: The well grown In vitro plantlets, after treatment with
0.2% (w/v) Bavistin1, were hardened by the following two-step procedure: (i) controlled
conditions of culture room for 3-4 weeks and (ii) semi controlled conditions in the shade
house for 2 weeks allowed 95% of plantlets to acclimatize, after which 99% of these
plantlets were surviving for 3 months after transfer to the field.