5. Conclusion
Tissue culture bananas can benefit from application of arbuscular
mycorrhizal fungi, Trichoderma and Bacillus to improve survival
and growth during the nursery phase as well as enhance performance
under field conditions. The effect of microbiological
inoculation is however dependent on soil type. The results from
this research reveal essential information in the management and
growth of tissue cultured banana and gives a possible strategy for
decentralization of commercial banana nurseries which depend
entirely on the use of conventional media. The results also confirm
the use of non-ammended soils for acclimatization as better and
more profitable as shown by our study since survival of inoculated
plantlets was enhanced by 33.0% above the non-inoculated controls
in the conventional media (only 57.0% of plants survived). This
means that for every 100 in vitro plants to be hardened, a farmer
using the natural soils will save 33 plants * I $USD =33$USD compared
to the farmer using conventional nursery media. However,
this is dependent on the composition of the microorganisms based
product applied. Further investigation should be carried out to verify
at what stage of plant development some of products should be
applied. For example, PHC Colonize was not suitable for hardening
but surviving inoculated plants performed well at the potting
stage. The knowledge generated on the effect of microbial inoculation
under field conditions is important in devising integrated
soil management options that enable optimal functioning of the
microorganisms. Soils with low phosphorous and potassium levels
limit root colonization, nutrient acquisition and subsequent plant
growth. Since no single productwasobserved to enhance all growth
parameters, combining of products is a necessary investigation.
There is limited information on combined application of microbial
inoculants (e.g. combining of mycorrhiza and Bacillus or Mycorrhiza
and Trichoderma) and the effect of their interaction on performance
of tissue culture banana under field conditions. These interactions
should therefore be explored for improvement of banana growth,
health and nutrition. The differential functioning of the mixed and
single species Bacillus and mycorrhizal based products revealed in
this study should be further investigated and considered in the
production of inoculum meant for specific soil conditions.
5. ConclusionTissue culture bananas can benefit from application of arbuscularmycorrhizal fungi, Trichoderma and Bacillus to improve survivaland growth during the nursery phase as well as enhance performanceunder field conditions. The effect of microbiologicalinoculation is however dependent on soil type. The results fromthis research reveal essential information in the management andgrowth of tissue cultured banana and gives a possible strategy fordecentralization of commercial banana nurseries which dependentirely on the use of conventional media. The results also confirmthe use of non-ammended soils for acclimatization as better andmore profitable as shown by our study since survival of inoculatedplantlets was enhanced by 33.0% above the non-inoculated controlsin the conventional media (only 57.0% of plants survived). Thismeans that for every 100 in vitro plants to be hardened, a farmerusing the natural soils will save 33 plants * I $USD =33$USD comparedto the farmer using conventional nursery media. However,this is dependent on the composition of the microorganisms basedproduct applied. Further investigation should be carried out to verifyat what stage of plant development some of products should beapplied. For example, PHC Colonize was not suitable for hardeningbut surviving inoculated plants performed well at the pottingstage. The knowledge generated on the effect of microbial inoculationunder field conditions is important in devising integratedsoil management options that enable optimal functioning of themicroorganisms. Soils with low phosphorous and potassium levelslimit root colonization, nutrient acquisition and subsequent plantgrowth. Since no single productwasobserved to enhance all growthparameters, combining of products is a necessary investigation.There is limited information on combined application of microbialinoculants (e.g. combining of mycorrhiza and Bacillus or Mycorrhizaand Trichoderma) and the effect of their interaction on performanceof tissue culture banana under field conditions. These interactionsshould therefore be explored for improvement of banana growth,health and nutrition. The differential functioning of the mixed andsingle species Bacillus and mycorrhizal based products revealed inthis study should be further investigated and considered in theproduction of inoculum meant for specific soil conditions.
การแปล กรุณารอสักครู่..