Currently, the identification of Vibrio species from clinical, environmental and seafood sources still relies on culture-based conventional diagnostics method. Although the conventional culture-based method is generally recognized as the classical method, it is laborious, time-consuming, and complex process involving the isolation of Vibrio species using commercial selective media followed by various biochemical and serological tests for identifying the presumptive colonies ( Neogi et al., 2010 and Yamazaki et al., 2008), and the specificity of using selective media is still questionable (Pinto, Ciccarese, Tantillo, Catalano, & Forte, 2005).