The PITCh system will be useful for a variety of applications, including creation of disease-modeling cells and animals for drug screening and therapy development. Additionally, it is expected that this knock-in technique will increase the production efficiency of useful recombinant proteins such as pharmaceutical materials in cultured animal cells. Furthermore, in silkworms, these strategies can be applied to production of functional recombinant silk proteins. The research group anticipates that the PITCh system will enhance the usefulness of genome editing techniques in a variety of cells and organisms, especially in those in which gene knock-in is hindered by low HR efficiency